The 134th Air Refueling Wing is made up of the 134th Operations Group, the 134th Maintenance Group, the 134th Mission Support Group, the 134th Medical Group and the 134th Wing Staff Agency.

McGhee Tyson is home to several tenant units including the 241st Engineering Installation Squadron, 119th Cyber Operations Squadron, I.G. Brown ANG Training and Education Center and 572d Air National Guard Band of the South.